Analysis Note

ControlBrain tissue

Application

Anti-Spinophilin/Neurabin-II Antibody is an antibody against Spinophilin/Neurabin-II for use in IC, IP & WB.

Research Sub CategorySynapse & Synaptic BiologyNeuronal & Glial Markers

Research CategoryNeuroscience

Immunoprecipitation:
4 µg of a previous lot immunoprecipitated Spinophilin/Neurabin-II from 500 µg of rat brain microsomal preparation (Catalog # 12-144).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Spinophilin/neurabin II is an actin-associated scaffolding protein enriched in the dendritic spines of neurons.

Immunogen

GST fusion protein corresponding to residues 286-390 of rat brain spinophilin/neurabin-II.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal IgG in buffer containing 70% storage buffer (0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide) and 30% glycerol. Store at -20°C.

Protein G Purified

Format: Purified

Quality

Routinely evaluated by Western Blot on a rat brain microsomal preparation.

Western Blot Analysis:
0.25-1 µg/mL of this lot detected Spinophilin/Neurabin-II in 20 µg of a rat brain microsomal preparation (Catalog # 12-144).

Specificity

Specific for spinophilin, Mr 120 kDa. Also recognizes a band which is suspected to be a cleavage product of spinophilin, Mr 95 kDa.

Other species not known.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

Target description

120 kDa